4 quick guide – reading a plate – Bio-Rad Model 680 Microplate Reader Accessories User Manual

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4.4

Quick Guide – Reading a plate

1. Turn on the instrument. Allow about 15 seconds for the self-diagnosis. Allow the

instrument to warm up for about three minutes prior to reading a plate.

2. Upon power up, the login screen will appear. Enter the login password (initial password:

00000), press Enter.

3. Program a protocol for your assay. See section 4.3.5 for editing a Kinetic protocol, or

section 4.3.7 for editing an End point protocol. Make sure filters installed correlate with
information entered in reader firmware.

4. Select the protocol of choice, if programmed. See the Memory Recall (section 4.3.4) for

detailed operation.

5. Review or print parameters set in the protocol. See section 4.3.7 Editing End Point

Protocol for details.

(a) Verify settings on Cutoff, Report, Limit, STDs, Mode, Mapping, and kit name

parameters are as desired.

(b) Protocol setting can be printed see section 4.3.3 Printing Reports and Protocol

information.

map with instructions as in section 4.3.11 Plate Map setting procedure.

(d) The Matrix and Limit reports require assignment of upper and lower limits. See

section 4.3.7, Editing End Point Protocol Limit setting window to set the upper
and lower limits.

(e) The Cutoff report requires definition of the cutoff setting. Refer to section 4.3.8

for cutoff setting procedure.

(f) The Curve fit and Concentration reports require that standard concentration and

locations be defined. Refer to section 4.3.9 for details to define the standards
and the curve fit parameters.

(g) Set general reading mode (single or dual wavelength reading, plate shaking,

read mode, and incubation) following instructions in section 4.3.10 Mode setting
procedure.

After warm-up is complete:

6. Press “Main” key, the main screen will appear on the LCD.

7. Carefully place the microplate in the reading chamber. Close the reading chamber door

and press the “Start/Stop” key to read.