Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 20)

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Negative cutoff = ( Negative Control average + 0.210) * 0.900
TeSeE eq:
Positive cutoff
= Negative Control average + 0.210
Negative cutoff = Negative Control average + 0.090
Samples are interpreted as follow:
“POS”: Positive when sample OD >= Positive cutoff
“NEG”: Negative when sample OD < Negative cutoff
“???”: Gray zone: NEG cutoff =< sample OD < POS cutoff
Reports of Kinetic Protocol
(1) Absorbance report
The Kinetic Absorbance report is the series of absorbance data in the kinetic
sequence.
(2) Kinetic Plots
The Kinetic plots are the absorbance plots of each well in the plate. It is available for
readers with the optional internal printer or with an external ESC/P printer which
accepts ESC/P code.
(3) Linear Regression report
The Linear Regression report provides the calculation of Kinetic reaction rate for
each well using the linear regression.
(4) GALT report
The GALT report calculates the following formula for each well between two
readings of a plate.
GALT = (R2 – R1) * k
Where:
R1 = Absorbance value of 1st reading
R2 = Absorbance value of 2nd reading
k = GALT factor
4.2.6 RS-232C
Interface
The Model 680 Microplate Reader has a built-in bi-directional RS-232C interface (9 pin D-sub
connector, Bio-Rad catalog number: 168-1005). This allows external computers to control the
instrument. When an external computer is in control of the microplate reader, the printer and
the membrane keys, except for the "Start/Stop" key, are automatically deactivated and the
LCD display as below.
The reader will remain in remote control mode until it receives "RL" command from the host
computer or until the "Start/Stop" key on the microplate reader is pressed.
Remote mode