Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 34)

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d. Cutoff Ratio setting
Ratio to Calibrator
In this screen, there are three items for setting the ratio to calibrator. The Up and
Down arrow key moves the cursor to the desired item in the menu list. The Enter
key seals the selection and resumes to the next setting screen.
Concentration of Calibrator
The Numerical keys and the Decimal point key are used to
enter the calibrator concentration value. The Enter key sets
the value and returns to the previous screen.
Unit of Calibrator
17 units are supported, as for the standard's unit. The unit
marked with “[ ]” is the current selection.
The setting procedure is the same as the unit selection of
Standards.
Cutoff value setting
This menu screen selects Single or Ranged cutoff type. The
cutoff type marked with “[ ]” is the current selection. The
Enter key seals the selection and resumes to the next setting
screen
Single Cutoff Control
The Numerical keys are used to enter the Positive cutoff
concentration value and gray zone value. The Enter key sets
these cutoff ratio values and returns to the previous screen.
Ranged Cutoff Control
The Numerical keys are used to input the Positive and the
Negative cutoff concentration value. The Enter key sets these
cutoff ratio and returns to the previous screen.
NOTE:
The calibrator concentration value is used to convert the
absorbance value of each well into concentration value. The
ratio of Concentration/Absorbance of Calibrator well is
applied to the conversion. The cutoff is done with the defined
Positive and Negative values to the converted concentration
value of each well.
Ratio to Calibrator
»■Concentration
•Unit
•Cutoff value
Concentration: 9999
unit: mol/l
↑»■ 01: ug/dl
02: ng/ml
03:[ pg/ml]
↓ 04: pm/ml
»■Positive >= 9999
Gray zone: 50 %
Unit: mol/l
»■Positive >= 9999
Negative < 9999
Negative
Unit: mol/l
»■Ranged cutoff
[Single cutoff]
Concentration
Unit
Cutoff value
Single cutoff
Ranged cutoff