Bio-Rad Image Lab™ Software - Manual (Page 80)

4 | Acquiring Images
80 | Gel Doc EZ Imaging System with Image Lab Software
weight. As an alternative, the point-to-point (semilog) method can be used
if the R
2
value is not sufficient.
 Fixed percentage gels: These gels have a nonlinear relationship between
the mobility and the molecular weight. For these gels, choose the logistic
or cubic spline regression method.
You can check how well each regression method fits the data in the standard curve
window (for more information, see Standard Curve on page 104 ). The linear
(semilog) regression method provides a measurement that describes how well the
standard curve fits the data R
2
value. The closer the R
2
value is to 1.0, the better the
data fit the standard curve.
The molecular weight of each band is displayed in the Analysis Table in the Mol. Wt.
column. Refer to Molecular Weight (MW) Analysis Tools on page 123 for more
information about molecular weight.
For information about the calculations behind the regression methods, see
Appendix E, Regression Calculation Methods .
Output Settings
Image Lab prints to the default printer unless you select otherwise.
You have three choices for specifying output:
 You can automatically print the image. This is useful for gel documentation,
especially if a thermal printer is connected. See Appendix F, Mitsubishi
P93/P95 Thermal Printer for instructions on setting up the Mitsubishi P93/
P95D thermal printer
 You can automatically print the report, including all accompanying analysis
information.
Note: You cannot print a report on a thermal printer.
 You can display the image on your computer.