4 acquiring images, Chapter 4, Acquiring images – Bio-Rad Image Lab™ Software User Manual
Page 69: Steps and choices. see, Chapter 4, acquiring images, 4acquiring images

User Guide
| 69
4
Acquiring Images
Acquiring an image requires a set of steps in a workflow called a protocol. In
Image Lab
software, a protocol can include any combination of settings for
imaging, analyzing, and reporting that has been saved to run as a single workflow.
Users can choose a protocol to be linked to a tray type by default. Such a default
protocol is associated with:
A specific type of tray
The green Run button on the front of the Gel Doc™ EZ imager
The user’s computer system login ID
The advantage of a default protocol is that it can be run with a single press of the
Run button on the imager. Custom protocols are run by clicking a software button in
Image Lab software. In all other respects, default protocols and custom protocols
are similar.
Typically, you create a default protocol for the applications you run most often with a
particular tray. Each user can set up four default protocols (one for each type of
sample tray) to run applications. For less frequently run applications, a custom
protocol can be created to capture the setup steps for later use.
After it is set up in Image Lab software, a default protocol is run by placing a gel in a
sample tray, inserting the sample tray in the imager, and pressing the Run button on
the front of the instrument. The imager automatically runs the default protocol
assigned to the type of tray inserted. If you have only one tray, it will automatically
be considered the default tray.