Bio-Rad Image Lab™ Software - Manual (Page 77)

Setting Up a Default Protocol
User Guide | 77
 Custom — select a numerical value between 1 and 100 to choose the best
detection sensitivity for your sample.
Note: When low or high band detection sensitivity is used, these numerical
values are set: low sensitivity = 25; high sensitivity = 75.
Step 3. Analyze Molecular Weight
1. To analyze molecular weight automatically, select the Analyze Molecular Weight
checkbox in the left pane of the Protocol Setup window. When this checkbox is
selected, the software calculates the molecular weight for each band based on
the specified standard.
2. To estimate the size of the molecules in the bands of your gel, enter the
standards you are using and specify the lanes in which the standards are
placed.