Bio-Rad Image Lab™ Software - Manual (Page 76)

4 | Acquiring Images
76 | Gel Doc EZ Imaging System with Image Lab Software
Analyze Image
Step 2. Detect Lanes and Bands
To analyze the gel automatically, Image Lab must detect lanes and bands on the
image. Lanes are detected automatically, and then the background is automatically
subtracted. Refer to Lane and Bands Tools on page 113 for details. You can
customize band detection with the following options.
Select the Lane and Band Detection checkbox in the left pane of the Protocol Setup
window.
The Protocol Setup window displays lane and band detection options:
 Low Band Detection Sensitivity — this option sets detection at a low
level for images with prominent bands. Faint bands are not detected.
 High Band Detection Sensitivity — this option sets detection at a higher
level for images that are faint. Extraneous bands can be removed later
using the Band Tools in the Analysis Tool Box. See Lane and Bands Tools
on page 113 .