Bio-Rad Image Lab™ Software - Manual (Page 200)

D | Using Bio-Rad’s Stain-Free Technology
200 | Gel Doc EZ Imaging System with Image Lab Software
The benefits of stain-free technology include:
 Elimination of staining and destaining steps for faster time to results
 No background variability within a gel or between gels (as is often seen
with standard Coomassie staining)
 Elimination of the need for acetic acid and methanol in staining and
destaining, which reduces organic waste
 Visualization of transferred or blotted proteins on low fluorescence PVDF
membranes
Stain-Free Workflow
For detailed information about the Activate/image gels step, refer to Acquiring
Images on page 69 . For all other workflow steps, refer to the Criterion™ Precast
Gels Instruction Manual and Application Guide (bulletin #4110001) or to the
Mini-PROTEAN® Precast Gels Instruction Manual and Application Guide
(bulletin #1658100).
Prepare buffers
Prepare gels and assemble
electrophoresis cell
Prepare and load samples
Perform electrophoresis
Activate/image gels
Analyze the separation