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Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System User Manual

Page 29

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Instruction

Manual | 21

20 | Automated Droplet Generator

2.4 Advanced Loading and Switching of Automated Droplet Generation Oils

Using the Automated Droplet Generator

2.4.1 Loading a Bottle of Oil before Configuring a Plate

To save time at the beginning of a run, a bottle of Automated Droplet Generation Oil can be loaded into the
instrument, and the oil delivery system primed, at any point. When there is no bottle of oil loaded into the
instrument, the Oil Level display on the screen will appear gray to indicate it is empty. The Oil Type display on
the screen will not display an oil type.

1. Open the door of the AutoDG Instrument by lifting up on the handle until it clicks into a locked position.

2. Remove the cap from the bottle of AutoDG Oil and insert the bottle into the tower of the oil delivery system

at the front left corner of the instrument.

3. Touch the Oil Type button on the home screen to bring up the Select Oil Type display.

4. Touch the droplet to indicate which type of oil has been loaded. To help differentiate, the AutoDG Oil for

Probes bottle has a yellow label and contains a yellow straw, while the AutoDG Oil for EvaGreen bottle has
a green label and contains a green straw.

Fig. 22. Home screen with oil level and oil type waiting to be set.

Fig. 23. A, oil selection display; B, AutoDG Oil for Probes selected.

A B