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Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System User Manual

Page 25

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Instruction

Manual | 17

16 | Automated Droplet Generator

Using the Automated Droplet Generator

2.2 Starting a Run from Idle Mode on the AutoDG

12. Once you have confirmed the plate setup, touch the Start Run button to begin droplet generation. The door

will automatically close at the beginning of the run and must remain closed during the run. Opening the door
before the Droplets Ready message appears will cause the instrument to terminate the run.

If the selected columns on the confirmation screen do not match the location of samples in your sample
plate, touch Back to change. Touch the sample plate to bring up the Configure Sample Plate window and
change your selection.

13. After a brief initialization, the Automated Droplet Generator will display a countdown timer on the screen

with time remaining until the plate of droplets is ready. No additional action is needed until the plate is
ready. The initialization can take 1–5 min, depending on whether the oil type has been changed or not
(see Section 2.4).

Fig. 16. The AutoDG door automatically closes at the start of each
run and must remain closed until droplet generation is complete.

Fig. 17. A, initializing display at the beginning of a run; B, countdown timer display indicates the time remaining until droplet
generation is complete and the Droplet Plate can be removed.

A B