Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System - Manual (Page 19)

Instruction Manual | 11 10 | Automated Droplet Generator
2.2 Starting a Run from Idle Mode on the AutoDG Using the Automated Droplet Generator
5. Open the door on the AutoDG Instrument by lifting up on the handle at the front of the instrument. The
electronic braking system will assist you in this task and prevent the lid from closing accidentally.
To avoid contamination, load the consumables from the back to the front of the instrument.
6. To load the DG32 ™ AutoDG Cartridges along the back row of the instrument:
a. Remove the plastic wrapping from the DG32 cartridges and place, with the green gaskets to the right,
into the three plate holders. The holders are keyed for proper orientation of each DG32 cartridge to
prevent incorrect loading.
b. The lights on the DG32 plate holders will change from yellow to green when the DG32 cartridges are
inserted correctly. If a light remains yellow, try repositioning the plate in a different orientation.
c. As the lights turn green on the deck, the corresponding icons on the touch screen will go from blinking
yellow to solid green, and Ready will be displayed.
The Automated Droplet Generator door will close to preserve HEPA-filtered enclosure if left
open for longer than 20 minutes while in idle mode (not generating droplets). There is an
audible click when the door brake releases, and the door closes slowly. Please observe the
pinch points of the instrument and keep hands clear.
Fig. 6. The Automated Droplet Generator keeps track of the consumables
so long as it is connected to a power supply. Based on the number of columns
configured, the system will prompt for only the number of consumables that need to
be loaded to complete the run. In this example, a full plate has been selected.