Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System - Manual (Page 28)

Instruction Manual | 21 20 | Automated Droplet Generator
Using the Automated Droplet Generator
2.3 Subsequent Steps
Once the 96-well plate containing the droplets is sealed, place it into the thermal cycler for PCR amplification.
Refer to the supermix product inserts for cycling conditions. When PCR amplification is complete, remove the
96-well plate from the thermal cycler and read the droplets using the QX100 or QX200 Droplet Reader (follow
the instructions in the QX200 Droplet Reader Instruction Manual, part number 10031906).
If the goal is to read or quantify droplets and recover material from droplets in parallel, prepare two sets
of reactions, one for each application. For example, a set of eight wells in a single DG8
™ cartridge can be
generated: four of these will be read after thermal cycling, and four will not be read. Refer to the QX200
Droplet Reader Instruction Manual (part number 10031906) for more details.
2.4 Advanced Loading and Switching of Automated Droplet
Generation Oils
When a new or different bottle of oil is loaded, or when power is lost, the Automated Droplet Generator will
perform a small volume flush and prime of the oil delivery system. If the AutoDG Instrument has not been used
for an extended time period (a week or more) but left powered on, it is recommended to perform a flush and
prime routine before running a plate.
Fig. 21. PX1 PCR Plate Sealer (left) and a sealed 96-well plate (right).
Begin thermal cycling (PCR) within 30 min of sealing the plate, or store the plate at 4°C for up to 4 hr prior
to thermal cycling. Refer to the supermix product inserts for cycling conditions.