Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System - Manual (Page 24)

Instruction Manual | 17 16 | Automated Droplet Generator
Using the Automated Droplet Generator
Automated Droplet Generation Oil for EvaGreen should be used only in combination with the
QX200
™ ddPCR system and ddPCR EvaGreen Supermix. The use of other supermixes can
lead to poor results and damage to the QX200 Droplet Reader. The QX100 ™ ddPCR System
does not support EvaGreen chemistry; running this chemistry will damage the instrument.
Fig. 14. Home screen; all consumables are correctly loaded and the
system is ready to start droplet generation.
11. Once all of the consumables are loaded and the corresponding lights are green on the deck and touch
screen, a blue Start button will appear at the bottom right of the screen. Touching Start will bring up a
confirmation window indicating the type of Droplet Generation Oil loaded, the number and orientation of
columns selected for droplet generation, and any plate name and details entered during configuration of
the sample plate.
A B C
Fig. 15. Run confirmation screen with A, full plate of samples selected; B, half plate of samples selected; C, alternating
columns of samples selected.