Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System - Manual (Page 21)

Instruction Manual | 13 12 | Automated Droplet Generator
Using the Automated Droplet Generator 2.2 Starting a Run from Idle Mode on the AutoDG
8. To load the 96-well PCR plate containing your prepared ddPCR reactions into the front row of the instrument:
a. The sample plate can be sealed with a PX1 ™ Plate Sealer and heat-sealing foil in advance of loading
into the AutoDG Instrument. Each well should contain 22 µl of your prepared ddPCR reaction. Please
see the supermix product insert for more detailed information on ddPCR reaction setup.
b. Place the plate into the front left plate holder, labeled on the screen as Sample Plate. The holder is
keyed for proper orientation and contains plate clips to support sealed plates.
c. The light on the Sample Plate holder will change from yellow to green when the plate is inserted
correctly. If the light remains yellow, try repositioning the plate in a different orientation.
d. As the light turns green on the deck, the corresponding icon on the touch screen will go from blinking
yellow to solid green, and Ready will be displayed.
Please note that a sample plate is required for every run, regardless of the number of columns selected.
Fig. 9. Pipet tip boxes have been correctly loaded. The Ready
status appears on the Pipet Tip icons on the screen.
Fig. 10. Sample plate has been correctly loaded. The Ready
status appears on the Sample Plate icon on the screen.