Bio-Rad QX200™ AutoDG™ Droplet Digital™ PCR System - Manual (Page 23)

Instruction Manual | 15 14 | Automated Droplet Generator
Using the Automated Droplet Generator 2.2 Starting a Run from Idle Mode on the AutoDG
If a bottle of Automated Droplet Generation Oil was previously loaded, you may be prompted to confirm the
type of oil currently loaded into the instrument.
If the last plate was run on the AutoDG Instrument with a different oil type than the one being currently
loaded and selected, the instrument will perform a small volume purge of the oil through the delivery
system and into the oil waste reservoir. The total run time will be a few minutes longer when this occurs,
but droplet generation will not be impacted. To prevent this operation in the future, see Section 2.4 on
advanced oil loading an switching.
10. To load Automated Droplet Generation Oil into the instrument:
a. Remove the cap from the bottle of Automated Droplet Generation Oil and twist the bottle into the tower
of the oil delivery system at the front left corner of the instrument.
b. Select the type of Automated Droplet Generation Oil that was loaded into the instrument by touching
the droplet for either Probes or EvaGreen oil. The droplet you select will turn blue; touch OK to set
the oil type.
c. The Oil Level icon on the screen will turn blue and display the current oil level of the bottle. The system
will display the oil type at the bottom left of the screen as well.
Fig. 13. Select oil type when loading a bottle of Automated
Droplet Generation Oil.
Why do I need a cooling block?
The AutoDG Instrument uses a cooling method to prevent droplet evaporation, much like the
Droplet Generator requires you to cover the wells once droplets are transferred.
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