Eppendorf BioSpectrometer fluorescence - Manual (Page 89)

89
Evaluation procedure
Eppendorf BioSpectrometer ® fluorescence
English (EN)
The factor is either entered directly as a parameter during the "Factor" evaluation procedure or calculated
during the "Standard" evaluation procedure (evaluation with a standard concentration):
F = calculated factor
C
S
= concentration of the standard (enter as parameter).
A
S
= measured absorbance of the standard.
If multiple measurement (2 or 3 replicates) has been programmed for the standard, the average value is
calculated from the measured absorbance values and inserted as A
S
.
12.3 Evaluation with standard curve/line
If evaluations are made with more than one standard, the following evaluation procedures for the standard
curve/line can be selected with the [Curve fit] in the
measure standards/new method step:
For the regression procedure, one can select that the regression line (regression curve) goes through the
zero point.
Evaluation procedure Description Minimum required number of
standard points
Linear interpolation Linear point-to-point connection
in the absorbance concentration
graph of the standard evaluation. 2 standards minimum.
Linear regression Polynome regression for first
degree polynomial. 3 standards minimum.
Quadratical regression Polynome regression for second
degree polynomial. 4 standards minimum.
Cubical regression Polynome regression for third
degree polynomial. 5 standards minimum.
Spline interpolation Interpolation via natural cubic
splines. 3 standards minimum.
S
S
A
C
F