Eppendorf BioSpectrometer fluorescence - Manual (Page 25)

25
Operation
Eppendorf BioSpectrometer ® fluorescence
English (EN)
1. Position the cuvette so that the optical window of the cuvette is pointing towards the direction of the
light path.
2. When inserting the cuvette, press it completely to the bottom against the slight resistance.
3. Fluorimetry: Close the cuvette shaft cover prior to measurement.
5.3 Summary of the measuring procedure
5.3.1 Preparing the measurement
1. Switch on the device and, if required, the printer.
The device performs a self test (taking approx. 1 minute) and displays the method selection.
2. Make ready the cuvettes for the measurements (see Inserting the cuvette on p. 24).
3. Prepare the measuring solutions for measuring the blank values, if required, also the standards and the
samples.
4. Open the cover of the cuvette shaft.
The direction of the light path is marked with an arrow on the housing.
• Photometry: The direction of the light path from back to front is marked on the housing:
"absorbance".
• Fluorimetry: The direction of the light path from right to left and back is marked on the
cuvette shaft cover: "fluorescence".
Abb. 5-2: Marking of light paths
Fig. 5-2: Marking of light paths
You should not use any measuring solution for standards and samples with a lower
absorbance than 0.02 to 0.03 A (e.g. dsDNA concentration between 1.0 and 1.5 μg/mL). The
detection limit of the device may be significantly lower, nevertheless, the impact of
disturbances from the measuring solutions (particles, bubbles, turbidity) on the reliability of
the result is very high for these low absorbance values.
a b s o r b a nce
h e i g h t
8 . 5 m m
fluorescence