Eppendorf BioSpectrometer fluorescence User Manual
Page 74
Troubleshooting
Eppendorf BioSpectrometer
®
fluorescence
English (EN)
74
The measuring results are
not correct.
• The method has not been
programmed correctly.
Ensure that the method parameters are
entered correctly.
• The standard solution has
not been prepared
correctly.
Ensure that the correct standard is used
and that the measuring solution for the
standard is prepared correctly.
• The absorbance of the
reagent is drifting.
For instable reagent absorbance and end
point methods: When measuring a long
series of samples, measure the reagent
blank value not only at the beginning but
also during the sample series. If the
reagent blank value drifts strongly, the
reagent is not appropriate for error-free
measurements and has to be replaced by a
new reagent.
• The cuvette is not
positioned correctly.
Position the cuvette in the cuvette shaft so
that the optical window points towards the
direction of the light path.
Photometry light path: from back to front
Fluorimetry light path: from right to left
Error
Possible cause
Remedy