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Eppendorf BioSpectrometer fluorescence User Manual

Page 48

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Methods
Eppendorf BioSpectrometer

®

fluorescence

English (EN)

48

Proteins (with
reagent)

Results display:

• Concentration result with

absorbance at the measuring
wavelength.

• For evaluation with standard

series: graph of the standard
evaluation with plotted sample
result.

Dye labels

Results display:

• Concentration results with

absorbance at the measuring
wavelength of the biomolecule.

• If activated in the parameters:

Scan.
Navigate between the measuring

points in the graph with and .

Additional data ([Data] softkey):.
If the corresponding parameters have
been activated:

• Ratios A260/A280 and A260/A230.
• Absorbance values for 280 nm and

230 nm and for the measuring
wavelength of the dye.

• FOI value.
• Absorbance values for the

background wavelengths.

Ratios and FOI are not displayed for
the measurement of the dye-labeled
proteins.

Bacterial density

Results display:

• Calculated result with absorbance

at the measuring wavelength.

• If activated in the parameters:

Scan.
Navigate between the measuring

points in the graph with and .

Method group
Photometry

Results display

Explanation