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Eppendorf BioSpectrometer fluorescence User Manual

Page 49

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49

Methods

Eppendorf BioSpectrometer

®

fluorescence

English (EN)

Basic

main group

Factor, standard

Analog to Protein direct UV (see above)

Results display:

• Concentration result with

absorbance at the measuring
wavelength.

Calibration curve Analog to Proteins (with reagent) (see above)

Results display:

• Concentration result with

absorbance at the measuring
wavelength.

• Graph of the standard evaluation

with plotted sample result.

Advanced

main group

Dual wavelength

Results display:

• Concentration result: calculated

from

A

calc.

with factor or standard

evaluation.

A

calc.

: calculated using the

formula, defined in the parameters,
created from the absorbances
measured on both wavelengths.

• Absorbance values that were

measured at the two measuring
wavelengths.

Additional data ([Data] softkey):.
If the corresponding parameters have
been activated:

• Absorbance value for the

background wavelength.

Method group
Fluorimetry

Results display

Explanation

Routine

main group

Nucleic acids

Results display:

• Concentration result with RFU

value at the measurement
wavelength

• Graph of the standard evaluation

with plotted sample result.

Method group
Photometry

Results display

Explanation