Eppendorf BioSpectrometer fluorescence - Manual (Page 84)

Technical data
Eppendorf BioSpectrometer
® fluorescence
English (EN)
84
11.4 Photometric properties
11.5 Fluorimeter
Measuring principle Single beam absorption spectrophotometer with reference beam
Light source Xenon flash lamp
Monochromator Holographic aberration-corrected concave grating
Beam receiver CMOS photodiode array
Wavelengths 200 nm to 830 nm
Wavelength selection Method-dependent, freely selectable
Spectral bandwidth ≤ 4 nm
Smallest step size 1 nm
Systematic wavelength error ±1 nm
Random wavelength error ≤ 0.5 nm
Photometric measuring range 0 A to 3.0 A at 260 nm
Reading accuracy ΔA = 0.001
Random photometric error ≤ 0.002 at A = 0
≤ 0.005 (0.5 %) at A = 1
Systematic photometric error ±1 % at A = 1
Stray light component < 0.05 %
Measuring principle Confocal filter fluorimeter with reference beam
Light source LED
Monochromator Filter arrangement consisting of dichroic and long pass filters
Beam receiver Photodiode
Excitation wavelength 470 nm
bandwidth: 25 nm
Emission wavelength I 520 nm
bandwidth: 15 nm
Emission wavelength Ii 560 nm
bandwidth: 40 nm
Measuring range 0.5 nM to 1 000 nM fluorescein (emission wavelength of 520 nm)
Random error of the
fluorimeter ±2 % at 1 nM fluorescein (emission wavelength of 520 nm)