Eppendorf BioSpectrometer fluorescence - Manual (Page 78)

Troubleshooting
Eppendorf BioSpectrometer
® fluorescence
English (EN)
78
9.3 Result flags
Warnings and error messages for results are displayed in the bottom right of the help box. The header bar
of the Help box is highlighted yellow for warnings and red for error messages.
Warnings: Decide whether the result is useful for you while taking the displayed warning into
consideration.
Error messages: No result is displayed; the reason is shown in the error message.
Problem Cause Solution
The standard curve is not
monotone. Please select
another Curve Fit. • No usable result was returned
during the evaluation of a standard
curve using the "spline
interpolation", "quadratic
regression" or "cubic regression"
Curve Fit procedures.
 Select a different Curve Fit
procedure.
Some absorbance values
for secondary
wavelengths are too high
or are not displayed. • For at least one secondary
wavelength, the absorbance
exceeded the measuring range.
• Secondary wavelengths are not
needed for calculating the
concentration result. They are used
for different purposes. For
example,
dsDNA method:
absorbance at 280 nm for the
calculation of
ratios 260/280.
• Turbidity of the measuring solution
• Measurements at the limits of the
photometric measuring range.
 If the absorbance values of the
secondary wavelengths are
relevant: Dilute the sample or
remove the turbidity via
centrifugation and repeat the
measurement.
The result is outside the
range of the standard
concentrations. • For methods with evaluation via
standard curves (nonlinear
evaluation method): The sample
result is up to 5 % outside of the
standard concentration range.
 Accept the measurement result, or
remeasure the sample under
conditions under which the result
is within the range of the standard
concentrations (dilute sample or
modify standard concentrations
and remeasure).