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Eppendorf BioSpectrometer fluorescence User Manual

Page 76

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Troubleshooting
Eppendorf BioSpectrometer

®

fluorescence

English (EN)

76

A method (or folder, dye,
protein, nucleic acid, or
unit) with this name
already exists.

• The name under which the method

was saved has already been used
for a different method in the same
folder.

• The message also appears after

editing names already given to a
folder or to a nucleic acid (dye,
protein, concentration unit) (under
General Method Parameter).

 Assign a different name.

The following parameter
values are not defined in
General Method
Parameter:

• When opening a method with

parameters which access

General

Method Parameter, the system
determined that at least one
parameter (dye, nucleic acid,
protein, unit) does not exist there
anymore, so probably has been
deleted.

 Select a different parameter from

the existing list. If necessary,
program a new list entry in
General Method Parameter in
order to be able to use it when
programming a method.

The value of the
parameter marked with

*

is not defined in the Gen.
Param. Please correct
the parameter.

This error message appears when
editing method parameters.

• Parameter in

General Method

Parameter is not defined.

 Select a different parameter from

the existing list. If necessary,
program a new list entry in
General Method Parameter in
order to be able to use it when
programming a method.

Invalid zoom interval.

During the Zoom process with free
entry of limits ([Free]) softkey):

• The zoom area is below the lower

limit.

 Enter the values so that the interval

does not fall below the range limits
of 0.02 A and 10 nm.

The entered standard
concentrations are not
monotonically increasing
resp. monotonically
falling. Correct the
standard concentrations.

• See the error text.

 Enter the standard concentrations

so that the first standard receives
the lowest concentration and the
other standard concentrations form
an increasing sequence.

At least two of the
entered standard
concentrations are
identical. Correct the
standard concentrations.

• See the error text.

 Enter the standard concentrations

so that the first standard receives
the lowest concentration and the
other standard concentrations form
an increasing sequence.

The measured values are
not strictly monotonous!

• Error when measuring a standard

series: The measured absorbance
values of the standard series are
not continuously increasing or
decreasing.

 Repeat the standard measurements

or delete the single, incorrectly
measured standard result.

Problem

Cause

Solution