Eppendorf BioSpectrometer fluorescence - Manual (Page 42)

Methods
Eppendorf BioSpectrometer
® fluorescence
English (EN)
42
6.5.2 Measure standards
Saving the method under a new name:
You can save the method in the same folder from
which you called up the method or in any folder in
the Favorites method group.
The name (maximum 20 letters) can be entered
using a displayed keyboard ([Keyboard] softkey) or
directly using the keyboard (see Entering text on
p. 23).
After saving you will return to the
check parameters
display.
The first standard to be measured is marked on the
display. After the blank value (
blank key) measure all
standards ( standard key) one by one.
When measuring more than one replicate per
standard, the average value for each standard is
calculated and displayed automatically.
By using the and cursor keys, you also can
select certain standards for measurement. Individual
standards can be remeasured as well.
Softkeys
• [Last cal]: Call up the last saved standard evaluation for this method in order to use it for sample
measurements.
• [Curve fit]: Select the procedure for the standard evaluation. If the result has not been saved, the
method can also be entered later. Instructions for selecting the evaluation procedure can be found in
the Evaluation procedure chapter (see Evaluation with standard curve/line on p. 89).
• [Graph]: Switch to the graphic display of the standard results.
As soon as the minimum number of results for the
evaluation with the selected method (curve fit) is
available, the evaluation result will be shown on the
right side of the display. You can now save the
evaluation and switch to sample measurements via
the [Next >] key.