Eppendorf BioSpectrometer fluorescence - Manual (Page 62)

Functions
Eppendorf BioSpectrometer
® fluorescence
English (EN)
62
When programming a method of the Dye labels or Proteins direct UV method groups, you can access the
entries in General Method Parameter:
Tab. 7-2: Parameter in General Method Parameter
 To edit a parameter group, use and to
select the parameter which you would like to
edit.
 Confirm with enter.
Softkeys
• [OK]: Save entry and return to the parameter group selection.
• [Cancel]: Return to the parameter group selection without making any changes.
Select the name of the dye to import the
corresponding parameter group into the method
program. By using the "edit" selection of the
"Nucleic acid" parameter, you also can get directly
to the
General Method Parameter function and
view and edit the parameters.
Parameter Explanation
Proteins These parameters are loaded into the method parameters when a protein
is selected during the programming of a method in the
Dye labels and
Proteins direct UV groups.
• Protein name
• Factor
• A
0.1%
• Ext.coeff.
• Molecular mass
In order to define a factor for calculating the concentration on the basis of
the absorbance, enter the following data in addition to the name and
wavelength:
Factor
or A
0.1%
or absorbance coefficient and molar mass.
Nucleic acids These parameters are loaded into the method parameters when a nucleic
acid is selected during the programming of a method in the
Dye labels
group.