Eppendorf BioSpectrometer fluorescence - Manual (Page 12)

Product description
Eppendorf BioSpectrometer
® fluorescence
English (EN)
12
2.3 Features
The BioSpectrometer fluorescence combines two spectroscopic measuring procedures: spectrophotometry
and fluorimetry. It is able to carry out both spectrophotometric measurements in the UV/Vis range of
200 nm to 830 nm and fluorimetric measurements at two defined wavelength combinations in the visible
range (470 nm excitation/520 nm emission and 470 nm excitation/560 nm emission). It is intended for the
measurement of liquids in cuvettes in development and research in the fields of molecular biology,
biotechnology, biochemistry and cell biology. You can use glass and plastic cuvettes in a volume range of
1 μL to 3000 μL (photometry) or 60 μL to 3000 μL (fluorimetry).
2.3.1 Methods
Photometry
Numerous methods for concentration determination of nucleic acids, proteins, and dye-marked nucleic
acids and proteins, and the
OD 600 method for determining bacterial density via turbidity measurement,
are already preprogrammed. Furthermore, method templates for various measurement and evaluation
procedures (single and multiple wavelength measurements, taking spectra, evaluations with factor,
standard and standard curve) are preprogrammed. It is possible to create individual methods on the basis
of the preprogrammed methods and templates. The templates in the Absorbance method group can be
used to quickly measure absorbances or spectra without an additional evaluation.
Fluorimetry
Methods for the concentration determination of nucleic acids with PicoGreen, RiboGreen, OliGreen and
Qubit reagents, and the concentration determination of proteins with NanoOrange, are preprogrammed.
For quick measurements with only two standards, short versions of nucleic acid methods are also included.
As in spectrophotometry, method templates are preprogrammed for various evaluation procedures (using
factor, standard, and standard curve).
2.3.2 Operation
The preprogrammed methods and templates are combined into clearly arranged groups from which the
desired method can be quickly selected. After calling up the method, you are guided through the
measuring procedure in clear steps. A help box in the display provides hints upon request. The 3 round
measuring keys (
standard, blank, sample) allow users to quickly start a measurement.
2.3.3 Result output
The BioSpectrometer fluorescence outputs the results using the device display and a printer available from
Eppendorf. With a USB connection, you can transfer result data from the device to a USB stick, a printer or
directly to a PC.
2.3.4 Device self test
The device automatically tests the function of the spectrometer unit and the fluorescence unit right after it
has been switched on. Access the
Device calibration function for a more comprehensive test (see Device
self-test on p. 71).