Eppendorf BioSpectrometer fluorescence User Manual
Page 80
Troubleshooting
Eppendorf BioSpectrometer
®
fluorescence
English (EN)
80
At least one of the results
is negative.
• For methods with several results
(e.g.,
Dye labels).
• Measuring solution not prepared
correctly.
• The incorrect factor has been
entered (wrong algebraic sign).
Measure again considering the
possible causes.
The result has more than
6 pre-decimal places.
• Very high sample concentration.
• Concentration unit does not match
the expected range of the sample
concentrations.
Dilute sample and measure again.
Change the concentration unit
(Parameter
Unit) and measure
again.
The result is more than
5 % outside of the
standard concentration
range.
• For methods with evaluation via
standard curves (nonlinear
evaluation method):
The sample result is more than
5 % outside of the standard
concentration range.
Remeasure the sample under
conditions under which the result
is within the range of the standard
concentrations (dilute sample,
modify standard concentrations
and remeasure).
• Calculation not
possible because of
division by zero.
Absorbance result is
zero.
• Calculation error.
Division by zero.
• The evaluation required dividing
by an absorbance result with the
value of "zero". This is not
mathematically permissible.
Examples: Calculation of a factor at
one-point calibration; calculation of
a 260/280 ratio with nucleic acid
measurements.
Check the reagents and samples
used and repeat the measurement.
Calculation not possible
because of division by
zero. Absorbance result
or parameter formula
b
is zero.
• An absorbance result was divided
by a "zero" value during the
evaluation of a
Division type
method (
Dual wavelength method
group). This is not mathematically
permissible.
Check the reagents and samples
used and repeat the measurement.
Do not enter "zero" as a value for
the Formula
b parameter.
Problem
Cause
Solution