Eppendorf BioSpectrometer fluorescence - Manual (Page 24)

Operation
Eppendorf BioSpectrometer
® fluorescence
English (EN)
24
5.2 Inserting the cuvette
Standard rectangular glass or plastic cuvettes can be inserted in the cuvette shaft:
• External dimensions: 12.5 mm × 12.5 mm
• Height of light path: 8.5 mm higher than cuvette base
• Total height: min. 36 mm
The cuvettes must be optically transparent for the respective measuring wavelength. For measurements in
the UV range, Eppendorf offers the plastic cuvette UVette which is transparent for wavelengths of 220 nm
and higher and therefore also is suitable for measuring nucleic acids.
Prerequisites
• The cuvette is free from contamination by dust or fingerprints and free from scratches.
• The cuvette shaft is free from particles, dust and liquid.
• The measuring volume in the cuvette is sufficient. Ensure that the minimum measuring volume has
been reached.
• The measuring solution is free from particles and bubbles.
• Fluorimetry: The measuring solution is free of substances that exhibit unwanted autofluorescence or
weaken the fluorescence of the substance to be examined.
• The cuvette temperature is above the temperature of the dew point that applies for the ambient
conditions (humidity and temperature).
Cuvettes
* or similar microliter cuvette
See manufacturer
information
not suitable
Macro Semi-micro Ultra-micro UVette ® Hellma ® TrayCell *
50 µL
60 µL
70 µL
70 µL
400 µL
400 µL
1000 µL
1000 µL
Eppendorf
µCuvette G1.0
Basic area 12.5 mm × 12.5 mm
See manufacturer
information
See manufacturer
information
Min. overall height 36 mm
Min. filling level 10 mm
Light path 8.5 mm
Max. height of base 7 mm
0 mm
Min. volume Photometry
Min. volume Fluorimetry