2 photometry method description, 1 absorbance method group, Photometry method description 6.2.1 – Eppendorf BioSpectrometer fluorescence User Manual
Page 32: Absorbance method group
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Methods
Eppendorf BioSpectrometer
®
fluorescence
English (EN)
32
Tab. 6-2:
Fluorimetric methods
You can create new methods in all folders using
In Favorites, you can create your own folders (e.g., to allocate folders to specific people), and rename and
delete the folders.
Tab. 6-3:
Softkeys in method selection
Copied or cut methods can be added to a different folder under Favorites, or added to the original folder
under a new name. Use the cursor keys to navigate to the
Methods column of the desired folder and press
[paste] for adding the method.
6.2
Photometry method description
The preprogrammed methods and method templates are described in this section.
6.2.1
Absorbance method group
Single λ
• Absorbance measurement on a wavelength.
• No subsequent evaluation.
Multi λ
• Absorbance measurement at two to six wavelengths.
• No subsequent evaluation.
Scan
• Absorbance wavelength spectra measurement via a defined wavelength range.
• Display of wavelength and absorbance in the spectrum by navigation with a wavelength cursor.
• The spectra section can be modified using 3 different zoom variants.
• Peak detection possible.
Routine
Fluorimetric nucleic acid and protein measurements with reagents
supplied by Invitrogen. (Carrying out these procedures may require a
license from Molecular Probes, Inc., Eugene, OR, USA or Invitrogen
Corporation, Carlsbad, CA, USA.)
Basic
• Methods for the evaluation of fluorescence measurements with
standard or standard curve/line.
•
Raw fluorescence method for rapid measurement of fluorescence
without further evaluation.
[Cut] and [Paste]
Cut and paste methods.
[Copy] and [Paste]
Copy and paste methods.
[Delete]
Delete methods.
[Rename]
Rename methods.