beautypg.com

2 photometry method description, 1 absorbance method group, Photometry method description 6.2.1 – Eppendorf BioSpectrometer fluorescence User Manual

Page 32: Absorbance method group

background image

Methods
Eppendorf BioSpectrometer

®

fluorescence

English (EN)

32

Tab. 6-2:

Fluorimetric methods

You can create new methods in all folders using

.

In Favorites, you can create your own folders (e.g., to allocate folders to specific people), and rename and
delete the folders.

Tab. 6-3:

Softkeys in method selection

Copied or cut methods can be added to a different folder under Favorites, or added to the original folder
under a new name. Use the cursor keys to navigate to the

Methods column of the desired folder and press

[paste] for adding the method.

6.2

Photometry method description

The preprogrammed methods and method templates are described in this section.

6.2.1

Absorbance method group

Single λ

• Absorbance measurement on a wavelength.
• No subsequent evaluation.

Multi λ

• Absorbance measurement at two to six wavelengths.
• No subsequent evaluation.

Scan

• Absorbance wavelength spectra measurement via a defined wavelength range.
• Display of wavelength and absorbance in the spectrum by navigation with a wavelength cursor.
• The spectra section can be modified using 3 different zoom variants.
• Peak detection possible.

Routine

Fluorimetric nucleic acid and protein measurements with reagents
supplied by Invitrogen. (Carrying out these procedures may require a
license from Molecular Probes, Inc., Eugene, OR, USA or Invitrogen
Corporation, Carlsbad, CA, USA.)

Basic

• Methods for the evaluation of fluorescence measurements with

standard or standard curve/line.

Raw fluorescence method for rapid measurement of fluorescence
without further evaluation.

[Cut] and [Paste]

Cut and paste methods.

[Copy] and [Paste]

Copy and paste methods.

[Delete]

Delete methods.

[Rename]

Rename methods.