Eppendorf BioSpectrometer fluorescence - Manual (Page 40)

Methods
Eppendorf BioSpectrometer
® fluorescence
English (EN)
40
6.5 Method procedure
FOI Selection:
none | dye/kb | pmole/
μg Only for the Dye labels method group.
Display of the FOI in addition to the result of the sample
measurement.
The FOI (frequency of incorporation) is a measure for the
number of dye molecules per nucleic acid molecule that are
integrated into the nucleic acid. Units are "dye/kb" (dye
molecules per 1000 bases) or "pmole/μg" (pmol dye per μg
nucleic acid). "None": no FOI calculation.
Background Selection:
on | off Prior to the calculation of the results of a sample the
absorbance of a background wavelength, during which the
analyte to be measured should exhibit the absorbance value
zero, is subtracted from the absorbance of the measuring
wavelength. Frequent application: Partial correction of
turbidity for measurement of nucleic acids (background
wavelength in this case: 320 nm or 340 nm).
Wavelength Wavelength in nm.
Range: 200 to 830 nm. Wavelength at which the background is to be measured. The
analyte to be measured should have the absorbance value zero
in pure form here.
Background for
dyes Selection:
on | off Only for the Dye labels method group.
Application of the background correction to the measurement
of a dye (see
Background parameter).
Wavelength Wavelength in nm.
Range: 200 to 830 nm. Only for the Dye labels method group.
Wavelength at which the background for the dye is to be
measured. The pure, not contaminated dye to be measured
should have the absorbance value zero for this wavelength.
Autoprint Selection:
on | off Printing a measuring result immediately following
measurement with the thermal printer.
Only the main result data will be printed. To output detailed
data, the required data packets can be compiled and printed in
the
print & export method step at the end of a measuring
series.
Wizard: the wizard at the top of the display will take
you through the method procedure. The currently
active method step is highlighted.
Parameter Entry Explanation