Eppendorf BioSpectrometer fluorescence - Manual (Page 71)

71
Maintenance
Eppendorf BioSpectrometer ® fluorescence
English (EN)
8.3.2 Checking the fluorescence unit
8.3.3 Device self-test
The frequency of the automatic self-test (approx. 1 minute) can be set using the Device settings function
(see Device settings on p. 64). The factory setting for Self-test interval is "Weekly".
The self-test checks the following:
• Verification of the detector
– Determination of the random error across the entire available spectrum
• Verification of the light source
– Verification of the maximum available energy of the light source and quality of the light transmission
through the device
– Determination of the signal random error at the reference sensor
– Determination of the signal level at the reference sensor
– Separate determination of light intensity in UV range
• Determination of the systematic and random error of the wavelength
– Position of an intensity peak in the UV range of the spectrum
– Precision of the position of an intensity peak in the UV range of the spectrum
1. Select the Fluorescence unit function in the
Device calibration group. Confirm with enter.
2. Place the F1 filter in the cuvette shaft. Press the
Measure softkey.
The device measures the test filter 15 times at 2
emission wavelengths. After the measurement
has been completed, the display shows 2
characteristic values: "Ratio" as the dimension for
the correct adjustment and "CV" as the dimension
for the noise.
3. Compare the characteristic values to the values in
the provided table. If the measured values do not
agree with the permitted range of values, contact
Eppendorf Service.