Luminex 100 IS User Manual, Version 2.3 - Manual (Page 89)

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MAP Technology Using Luminex 100 IS 2.3 Software
PN 89-00002-00-071 Rev. C 5 - 47
Ensure that the Luminex analyzer lasers are warmed up and the
probe height is set correctly before calibrating the system. Do not
move the system waste line while calibrating.
You can run calibration and verification commands from the
Maintenance tab. You can import and export calibration and control
lots and reuse existing lot information for calibration and controls.
Run System xMAP
Calibrators
‹
To calibrate your system with xMAP calibrators:
1. Vortex the xMAP calibrator and control containers to ensure
homogeneity. Do not dilute xMAP calibrator or control reagents.
2. Load a microtiter plate with at least five drops of each: CAL1 in
well A1, CAL2 in well B1, CON1 in well C1, CON2 in well D1
and distilled water in well E1 through H1 to wash a total of four
times. Use different wells as necessary. To select different well
locations in the software, click on the drop-down arrow next to
the entry cell for the calibrator or control, then click in the well
location on the microtiter plate image.
3. Click Eject/Retract, then place the plate on the plate holder.
4. Fill the Luminex XYP reservoir with a solution of 70%
isopropanol or 70% ethanol.
5. Click Eject/Retract.
6. On the Maintenance tab, click Prime. Click OK and wait for
the Prime to finish (about 1½ minutes).
7. Click Alcohol Flush. Click OK and wait until the alcohol flush
completes. The Device Status section in the status bar changes
from yellow to green and displays “Standby”.
8. Click New CAL Targ. to enter or confirm calibration lot
numbers in the Update CAL Targets dialog box. See Figure 5-
25.
9. Enter the CAL1 lot number and expiration date as printed on the
bottle. Enter the values listed on the Certificates of Quality
(COQ) included with your calibrators into the CAL1 boxes. If
you are using a previously entered lot, select it from the drop-
down menu in the Current CAL1 group box or click Import to
import the information. See page 5-50 for more details on
importing a Calibration lot.
Note: When dispensing calibra-
tion and control microspheres,
hold the bottle upside down at
a 90-degree angle to the micro-
titer plate to ensure that you are
getting accurate drop volume.