Luminex 100 IS Version 2.1 - Manual (Page 71)

PN 89-00002-00-062 Rev. A 65
xMAP Protocols
Updates or additions to these protocols are posted on the Luminex
website at
http://www.luminexcorp.com . At he home page, select
select Customer Support.
The protocols in this appendix are presented in the following order:
‰ Two-Step Carbodiimide Coupling of Protein to xMAP
Carboxylated Microspheres
‰ One-Step Carbodiimide Coupling of Oligonucleotides to xMAP
Carboxylated Microspheres
‰ Binding Biotin-conjugated Molecules to xMAP
LumAvidin
®
-Modified Microspheres
xMAP Microsphere Handling
xMAP Microsphere
Dispersion
Luminex xMAP microspheres settle and aggregate if left
undisturbed. You should always ensure that they are evenly
distributed before dispensing. Gentle vortexing and sonication are an
effective method of mixing for most coated microsphere
preparations. After dispersion, microspheres generally remain in
solution for about one hour. To maintain bead concentration within
the stock microsphere volumes (1 mL, 4 mL, or 16 mL), we suggest
that you follow these guidelines.
For complete resuspension of the 1 mL stock vials, Luminex
recommends centrifuging, sonicating, and vortexing. Failure to do so
often results in decreased amounts of microspheres recovered in
future sample aliquots.
Larger 4 mL and 16 mL vials should be placed on a rotator for a
minimum of 15 minutes for complete resuspension. Vortexing and
B