Luminex 100 IS Version 2.1 - Manual (Page 13)

x MAP Technology Luminex xMAP Technology
PN 89-00002-00-062 Rev. A 7
The volume restrictions that the amount expelled back function
places on the assay design can be expounded by the following
formula.
Sample Timeout
Sample Timeout is the amount of time in seconds that the instrument
will read the sample to detect the specified number of events.
Specify a timeout for assay wells. If sufficient events are not
collected in the specified time, the Luminex 100 IS logs an error,
flags the sample, and goes on to the next well. Set sample timeout to
several times longer than the expected read time of the well. Set to
zero to not use the timeout property.
Sample Dilution
Serum samples must be diluted at least 1:5 through dilution with
reagents as part of assay setup or as a final dilution step.
Reagents
Formulated reagents must be free of particulates other than
microspheres.
Kit Instructions
When you develop kit instructions, include:
•
Include dilution
• Preparation steps, such as sample probe height adjustments that
need to accommodate the selection of plate type
• XYP heater temperature setting
• Information about assay controls and standard values for each
test in the assay as product lot information
• Template name and version number in the kit instructions
• Template provided on a 3½” diskette or CD
[“Total well volume” (uL ) – “Sample uptake volume”(uL)] + 160(uL)
Where:
Total well volume The starting sample volume of a well
before the unit samples for acquisition.
Sample uptake
volume The volume the unit uptakes for
acquisition (program this in the template
as sample vol.)
160uL The amount expelled back volume stated
in this section.
Maximum Well
volume plate The maximum volume capacity of the
user selected 96 well microtiter plate.