Luminex 100 IS Version 2.1 - Manual (Page 11)

x MAP Technology Luminex xMAP Technology
PN 89-00002-00-062 Rev. A 5
microspheres are not monodispersed and tend to aggregate until
coated. Multiple pipetting from the original container may affect
bead concentrations. Protect the microspheres from light at all times.
Follow the resuspension instructions based on the size of container in
use:
For 1 mL containers: Remove from 2-8°C and allow equilibration to
room temperature. Centrifuge for 1-2 minutes at ≥ 8,000 x g.
Sonicate in a water bath sonicator to disperse the xMAP microsphere
pellet. Mix by gentle vortexing immediately prior to dispensing.
Inversion is not recommended.
For larger containers: Remove from 2-8°C and allow equilibration to
room temperature. Place on rotator and rotate for a minimum of 15
minutes. Mix by gentle inversion immediately prior to dispensing.
For these containers, vortexing and rocking are not recommended.
Bead Selection
You select beads from the standard 100-region bead map. Refer to
Table 1. Luminex recommends that you select beads in this order,
starting from the upper left, reading across to the end of a row, and
then down to the next row:
Bead Concentration
To a great extent, the concentration of beads in an assay determines
system speed. To analyze a 96-well plate in one hour, we recommend
you use at least 5000 beads per target and do not exceed a bead
concentration of 2.5 x 10
6 microspheres/mL.
Table 1: Bead Selection
033 034 035 036 037 038 042 043 044 045
046 047 051 052 053 054 055 056 061 062
063 064 065 066 072 073 074 075 076 077
017 018 019 020 021 024 025 026 027 028
029 032 041 011 012 013 014 006 007 008
083 084 085 086 087 088 089 090 048 057
058 067 068 069 078 079 080 002 003 004
009 015 022 030 039 049 050 059 060 070
071 081 082 001 005 010 016 023 031 040
091 092 093 094 095 096 097 098 099 100