Luminex 100 IS Version 2.3 - Manual (Page 173)

x
MAP Technology Troubleshooting
PN 89-00002-00-072 Rev. C 7 - 19
Verification
‹
To verify your system from the Maintenance tab:
1. Vortex xMAP reagent containers to ensure homogeneity.
2. Load a microtiter plate with CON1, CON2, and four wells with
sheath fluid in the six desired wells.
3. Click Eject/Retract. The plate holder ejects.
4. Place the plate on the plate holder.
5. Fill the Luminex XYP reservoir with a solution of 70%
isopropanol or 70% ethanol.
6. Click Eject/Retract. The plate holder retracts.
7. If you are using a new lot, click New Control Targets from the
Maintenance tab, fill in the required information, and click OK.
8. Click Prime. The Confirmation dialog box opens. Click OK to
begin priming. Wait until the Prime is completed.
9. Click Eject/Retract to eject the Luminex XYP instrument tray.
Place 70% isopropanol or 70% ethanol in the reservoir. Click
Alcohol Flush. The Confirmation dialog box opens. Click OK
to begin the Alcohol Flush.
10. Click OK and wait until the alcohol flush completes. The Device
Status section on the Status Bar changes from yellow to green
and indicates Standby. This takes about five minutes.
11. Select the well where CON1 is located from Step 2 using the
drop-down arrow located to the right of the CON1 button. Verify
the location. Click CON1. The Confirmation Screen dialog box
opens. Click OK. Wait until CON1 completes.
12. Select the well where CON2 is located from Step 2 using the
drop-down arrow located to the right of the CON2 button. Verify
the location. Click CON2. The Confirmation Screen dialog box
opens. Click OK. Wait until CON2 completes.
13. Verify that the system controls completed successfully from the
Diagnostics tab System Monitor. If controls are successful, the
date and time appear as green text. If controls fail, the date and
time are appear as red text.
14. On the Maintenance tab, click the well where you placed the
sheath fluid. Click Wash. A confirmation dialog box opens.