Drucker Diagnostics Paralens Advance - Manual (Page 7)

Section 1
Introduction
1.3 PRINCIPLES OF THE PROCEDURE
QBC hematology tests utilize precision-bore glass
tubes pre-coated with potassium oxalate, acridine
orange fluorochrome stain,
7 and an agglutinating
agent. QBC tubes made specifically for capillary
blood (finger-stick samples) additionally contain
a coating of anti-coagulants. During high-speed
centrifugation of the blood-filled tube, the cells
form in packed layers around the float, which has
descended into the buffy coat (Figure 1-3).
8
The Autoread Plus analyzer accommodates all QBC
tube types. The spun tube is inserted in the analyzer,
where it is automatically scanned and fluorescence
and absorbance readings are made to identify the
expanded layers of
differentiated cells. Volumes
of these packed cell layers are then computed to
obtain quantitative values of the following:
• Hematocrit
• Hemoglobin
• Mean Corpuscular Hemoglobin Concentration
• Platelet Count
• White Blood Cell Count
• Granulocyte Count (% and number)
• Lymphocyte-Monocyte Count (% and number)
Hemoglobin is computed from density factors
that determine its cellular concentration. Mean
corpuscular hemoglobin concentration is
calculated electronically according to the equation:
MCHC = (HB ÷ HCT) × 100.
1.4 WARNINGS AND PRECAUTIONS
The QBC Autoread Plus Hematology System is
intended for in vitro diagnostic use.
Carefully observe all warnings and precautions
in this manual and on labeling of QBC tubes
concerning the safe handling of blood and blood-
derived products.
Figure 1-3.
Color-Separated Layers
Spun QBC Blood Tube
WARNING
Acridine orange reagent may be toxic; do not
ingest. Avoid contact with skin, eyes, and
clothing.
WARNING
Blood specimens may contain the Hepatitis B
Virus (HBV), Hepatitis C Virus (HCV), Human
Immuno deficiency Virus (HIV), or other disease-
causing agents. Handle all patient specimens
as potential biohazards capable of transmitting
infection. Wear appropriate personal protective
equipment, including gloves, when collecting
and processing blood.
WARNING
QBC blood tubes are made of glass. Be
careful when handling and preparing tubes to
prevent breakage and possible injury. Inspect
QBC tubes before use. Do not use cracked or
scratched tubes.
1-2
PLASMA
FLOAT
RED CELLS
AROUND
FLOAT
STOPPER
PLATELET LAYER
WHITE CELLS (LYMPHOCYTES &
MONCYTES)
WHITE CELLS (GRANULOCYTES)
RED CELLS