Drucker Diagnostics Paralens Advance - Manual (Page 39)

Section 5
Specimen Collection and Preparation for Testing
5.1 VENOUS BLOOD FOR HEMATOLOGY
Draw venous blood into collection tubes containing
the anticoagulants disodium or tri-potassium
ethylenediamine-tetraacetate (EDTA).
5.1.1 Collection Procedures
• Be sure to wear laboratory gloves. Use only
clean glassware and sterilized collecting
instruments. Before venipuncture, clean the
skin area with an antiseptic agent and wipe dry.
• Draw venous blood with a Vacutainer™
brand blood collection tube* or other blood-
drawing device containing EDTA. To assure an
acceptable blood-to-anticoagulant ratio, fill the
collection tube to at least 2/3 of its fill volume.
Remove the collection needle and dispose
properly in a sharps container.
• Thoroughly mix the blood with the anti coagulant.
If clots are present, discard the specimen.
5.1.2 Anticoagulants
Always anticoagulate venous blood with disodium
or tri-potassium EDTA. The use of other
anti coagulants is not recommended.
5.1.3 Interfering Substances – QBC AccuTubes
• Hemolysis: Do not perform tests on visibly
hemolyzed blood specimens.
• Bilirubin: No effects on test results have been
observed at biliruben concentrations up to
20 mg/dL. 9
• Triglycerides: No effects on test results have
been observed at triglycerides concentrations
up to 1,800 mg/dL. 9
• Coumadin: Anticoagulant therapy has been
shown to have no clinically significant effect on
performance. 9
• Doxorubicin: Treatment with the anthracyclic
drug Doxorubicin does not appear to interfere
with the QBC test method. 9
• Other drugs: The effects of other potentially
interfering drugs and their metabolites on QBC
tests have not been established. 10,11
Interfering substances information for all other QBC
tube types can be found in Appendix A-1.
WARNING
Blood specimens may contain the Hepatitis B
Virus (HBV), Hepatitis C Virus (HCV), Human
Immuno deficiency Virus (HIV), or other disease-
causing agents. Handle all patient specimens
as potential biohazards capable of transmitting
infection. Wear appropriate personal protective
equipment, including gloves, when collecting
and processing blood.
*Product of BD Vacutainer Systems
5-1