LaMotte SMART3 Colorimeter - Manual (Page 54)

 ENTERING A MULTIPLE POINT CALIBRATION
The SMART3 can directly create a 2 point calibration curve. (See Entering a
Two Point Calibration on page 47.) To create a multiple point calibration curve,
constants obtained from a linear regression of multiple data points can be
entered into the SMART 3.
1. Scan reactions of multiple concentrations at the appropriate wavelength in the
absorbance mode on the SMART3.
2. Plot the concentration (y axis) versus absorbance (x axis) in a program
capable of linear regression such as Excel.
3. Enter the constants obtained from the linear regression equation into the
SMART3.
For Example:
y = 0.001x 3 – 0.017x 2 + 0.181x – 0.049
K0 = – 0.049
K1 = 0.181
K2 = – 0.017
K3 = 0.001
OR (Over Range) = 10
1. From the User Test menu,
press or to scroll
to Enter Constants.
111 H2O
Name the Test
Select Vial/WL
STD Calibration
Enter Constants
12:00:00 001/500
2. Press
ENTER
to select Enter
Constants.
K0=0.00000
K1=0.00000
K2=0.00000
K3=0.00000
OR=100.00000
12:00:00 001/500
52 SMART3 Colorimeter 11.10
Editing/Set Up