LaMotte SMART3 Colorimeter - Manual (Page 13)

 AN INTRODUCTION TO COLORIMETRIC ANALYSIS
Most test substances in water are colorless and undetectable to the human
eye. To test for their presence we must fi nd a way to “see” them. The SMART3
Colorimeter can be used to measure any test substance that is itself colored or
can be reacted to produce a color. In fact a simple defi nition of colorimetry is
“the measurement of color” and a colorimetric method is “any technique used
to evaluate an unknown color in reference to known colors”. In a colorimetric
chemical test the intensity of the color from the reaction must be proportional
to the concentration of the substance being tested. Some reactions have
limitations or variances inherent to them that may give misleading results. Many
such interferences are discussed with each particular test instruction. In the
most basic colorimetric method the reacted test sample is visually compared to
a known color standard. However, accurate and reproducible results are limited
by the eyesight of the analyst, inconsistencies in the light sources, and the
fading of color standards.
To avoid these sources of error, a colorimeter can be used to photoelectrically
measure the amount of colored light absorbed by a colored sample in reference
to a colorless sample (blank).
White light is made up of many different colors or wavelengths of light. A
colored sample typically absorbs only one color or one band of wavelengths
from the white light. Only a small difference would be measured between white
light before it passes through a colored sample versus after it passes through
a colored sample. The reason for this is that the one color absorbed by the
sample is only a small portion of the total amount of light passing through the
sample. However, if we could select only that one color or band of wavelengths
of light to which the test sample is most sensitive, we would see a large
difference between the light before it passes through the sample and after it
passes through the sample.
The SMART3 Colorimeter passes one of four colored light beams through
one of four optical fi lters which transmits only one particular color or band
of wavelengths of light to the photodectector where it is measured. The
difference in the amount of colored light transmitted by a colored sample is a
measurement of the amount of colored light absorbed by the sample. In most
colorimetric tests the amount of colored light absorbed is directly proportional
to the concentration of the test factor producing the color and the path length
through the sample. However, for some tests the amount of colored light
absorbed is inversely proportional to the concentration.
The choice of the correct wavelength for testing is important. It is interesting to
note that the wavelength that gives the most sensitivity (lower detection limit)
for a test factor is the complementary color of the test sample. For example the
Nitrate-Nitrogen test produces a pink color proportional to the nitrate-nitrogen
concentration in the sample (the greater the nitrate-nitrogen concentration, the
darker the pink color). A wavelength in the green region should be selected to
analyze this sample since a pinkish-red solution absorbs mostly green light.
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