LaMotte SMART3 Colorimeter - Manual (Page 17)

PREPARING DILUTE STANDARD SOLUTIONS
Standard solutions should be prepared to create a calibration curve. Standard
solutions can be prepared by diluting a known concentrated standard by
specifi ed amounts. A chart or computer spreadsheet can be created to
determine the proper dilutions. Use volumetric fl asks and volumetric pipets for
all dilutions.
1. In Column A – Record the maximum concentration of test as determined by
the range and path length.
2. In Column B – Record the percent of the maximum concentration the
standard solution will be.
3. In Column C – Calculate the fi nal concentration of the diluted standard
solutions by multiplying the maximum concentration (In Column A) by the %
of maximum concentration divided by 100. (C = A x
B
/
100
).
4. In Column D – Record the fi nal volume of the diluted sample (i.e. volume of
volumetric fl ask).
5. In Column E – Record the concentration of the original standard.
6. In Column F – Calculate the milliliters of original standard required (F = (C x
D
/
E
)).
A sample chart appears below:
A B C =
A x
B
/
100
D E F =
C x
D
/
E
Maximum
concentration
of test
% of Maximum
concentration
Final
concentration
of Diluted
Standard
Volume of
Standard
Concentration
of Original
Standard
mL of
Original
Standard
Required
10.0 ppm 90 9.0 ppm 100 mL 1000 ppm 0.90 mL
10.0 ppm 70 7.0 ppm 100 mL 1000 ppm 0.70 mL
10.0 ppm 50 5.0 ppm 100 mL 1000 ppm 0.50 mL
10.0 ppm 30 3.0 ppm 100 mL 1000 ppm 0.30 mL
10.0 ppm 10 1.0 ppm 100 mL 1000 ppm 0.10 mL
10.0 ppm 0 0 ppm 100 mL 1000 ppm 0 mL
SMART3 Colorimeter 11.10 15