Eppendorf FemtoJet - Manual (Page 32)

30
5 Troubleshooting
5.1 Potential errors
– Pressure loss due to capillary not fitted with capillary holder
connected.
Solution: Connect the capillary or select Function 0, Change capillary,
via the key and variable regulator p
i
.
– Capillary is blocked.
Solution: Press the key for long enough.
Change capillary.
Purify injection fluid (e.g. by means of centrifugation).
– Compressor runs for a long time.
Solution: Take a short break.
Do not use the key too often within a short period.
If possible, set a lower compensation pressure p
c.
Perform self-test (Section 5.1.1).
– Valve rattles
Solution: Connect tube to filled capillary. Pressure control is designed
for the tube connected.
– No communication with the micromanipulator.
Solution: Check cables and connections.
5.1.1 Self-test
FUNC 6
The FemtoJet

is checked for leaks in
the self-test.
This self-test can also be used if a leak is
suspected.
The test runs automatically.
The injection tube must be connected to
a closed capillary (e.g. melted closed or
filled) for the pressure control to work
properly.
FUNC 6
Self-Test
INJECT = Complete
CLEAN = Leak only
Turn pi to
select function
5 Troubleshooting
05_Fehler_en.fm Seite 30 Mittwoch, 27. September 2006 5:14 17