Eppendorf FemtoJet - Manual (Page 22)

20
3 Mode of operation
The usual values for the permanent compensation pressure P
c
are bet-
ween 30 – 50 hPa (0,44 – 0,73 PSI) for Femtotips and similar capillaries.
The FemtoJet shows good, constant values within this range Immediately
after the device is switched on and maintains the balance between
undesired absorption of medium on the one hand and excessive loss of
the liquid to be injected on the other hand.
Quite low P
c
values are sometimes advisable for special applications
(e.g. capillaries with an interior diameter of 5 µm and greater)! In order to
be able to also work precisely and efficiently here, the FemtoJet should be
switched on app. 60 to 90 minutes before beginning the experiment to
guarantee the correct thermal settings. A pressure setting below 5 hPa is
not possible. You may merely also set 0 hPa.
An internal self-calibration of 3 seconds for more precise adjustment of
the pressure takes place every 10 minutes. No injection is carried out
during this time.
Injection can continue as usual following the self-calibration.
An already activated injection will be carried out by the FemtoJet. The
self-calibration takes place afterward.
Message during the self-calibration on the display of the FemtoJet:
If the FemtoJet is connected with the InjectMan NI 2, the following
message appears on the display of the micromanipulator:
Self-calibration intervals:
– In the first hour after the machine is switched on: every 10 minutes.
– Afterwards: Every 30 min at pc < 30 hPa or every 15 min at pc > 30 hPa
following the last activation.
A brief signal sounds at the beginning
and end of the calibration.
Press the joystick button again to repeat
the injection.
WAIT
self calibration
Warning 001
Check Injector and cable
Press joystick button
03_Bedien_en.fm Seite 20 Mittwoch, 27. September 2006 5:12 17