Eppendorf FemtoJet - Manual (Page 25)

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3 Mode of operation
3.4.5 Manual or automatic injection
In the Manu operating mode, injection is performed for as long as the
key or hand / foot control is held down. The duration (t
i
) of
injection is measured and shown in the display. In the case of
combination with an Eppendorf Micromanipulator, the injection movement
is triggered.
In the Auto operating mode, the built-in timer facilitates a reproducible
injection time. The injection runs automatically for the duration of set time
t
i
. It is triggered by the key or the hand / foot control being pressed
briefly. During injection, the Inject key is inactive, i.e. pressing it again has
no effect. In combination with an Eppendorf Micromanipulator, the auto-
matic injection procedure can also be triggered from the operating panel
of the micromanipulator.
3.4.6 Constant pressure/constant working pressure
Using the Constant Pressure function, it is possible to select a higher
pressure permanently if required. This function is helpful when special
injection techniques require either a high pressure up to a maximum of
6,000 hPa (87 psi) or a permanent flow of injection solution
(see Section 3.7.5). Control accuracy drops in the pressure range from
2,000 – 6,000 hPa (29 – 87 psi).
3.4.7 General note
FemtoJet

is fitted with an integrated compressor. For this reason, the
compressor may start up or excess pressure may be let off briefly before,
during and after injections or during periods when the FemtoJet

is not
being used. This is not a malfunction of the device and does not impair
injection.
In the case of injections under high pressure at very short intervals
or in the case of the Clean function being carried out several times
in succession, the pressure in the system accumulator may drop
significantly. This is indicated by a warning or an error message. In this
case, wait a short time until the supply pressure has stabilized again.
It is then possible to continue working immediately.
03_Bedien_en.fm Seite 23 Mittwoch, 27. September 2006 5:12 17