Eppendorf FemtoJet - Manual (Page 14)

12
3 Mode of operation
3.1 Display, variable regulators and keypad
Before operating the device for the first time, users should read this
section carefully. This section describes operation of the device only.
Handling of the injection capillaries is described in Section 3.3.
Display
The display shows the following:
1st line:
injection mode AUTO or MANU,
counter n for number of injections
performed
2nd line (bold):
The most-recently set parameter or
relevant parameter
Bottom lines:
Parameters which affect injection
Compensation pressure p
c
which is
applied permanently in the normal
mode is displayed as an actual
measured value.
The arrangement of the three
parameters shown, p
i
, t
i
, p
c
,
corresponds to the arrangement of
the variable regulators.
3.1.1 Setting the injection parameters
The injection parameters have to be adapted to the injection capillaries
used and the cell type and liquid which are to be injected.
The following injection parameters can be set using the variable
regulators:
Injection pressure p
i
0; 5 – 6,000 hPa (0.00; 0.07 – 87.0 psi)
Injection time t
i
0.1 – 99.9 s
Compensation pressure p
c
0; 5 – 6,000 hPa (0,00; 0.07 – 87.0 psi)
The settings are in increments of 1 hPa/0.1 s. The display can show hPa
or psi, as required. The setting and internal pressure conversion are
always performed in hPa. Rounding for the display in psi can result in
increments of 0.01 to 0.02 psi.
The display of pressure in psi varies according to the size of the value
(e.g.: 9.99 may also appear in the display as 10.0).
AUTO n= 0
pi= 150 hPa
pi[hPa] ti[s] pc[hPa]
150 0.2 50
AUTO n= 0
pi= 215 PSI
pi[PSI] ti[s] pc[PSI]
2.15 0.2 0.72
3 Mode of operation
03_Bedien_en.fm Seite 12 Mittwoch, 27. September 2006 5:12 17