Eppendorf FemtoJet - Manual (Page 24)

22
3 Mode of operation
3.4.3 Injection time t
i
Injection time t
i
gives the period for which injection pressure p
i
is
maintained. t
i
is usually selected from a range between approx. 0.3 s and
1.5 s. The relevant factor for the injected volume is the product of the
injection pressure and the injection time.
Depending on the setting on the Eppendorf Micromanipulator which is
connected, the injection time t
i
is measured from the point at which the
key is pressed. The injection pressure is built up immediately, so it
is already present during the injection movement and during penetration
of the cell. Once the end of injection time has been reached, the device
switches to compensation pressure p
c
. At the same time, the micromani-
pulator is moved back into the working level. When "Injection at Z limit" is
set, measurement of the injection time t
i
does not begin until the Z limit
has been reached. Once t
i
has elapsed, the FemtoJet

switches back to
p
c
and the micromanipulator moves back into its starting position in the
working level.
3.4.4 Clean function
The Clean function is used to flush out blocked capillaries. The maximum
available pressure is applied to the outlet with the key. It is applied
for as long as the key is pressed. The maximum pressure of the
FemtoJet

is in the range of 7,000 hPa (102 psi). The value cannot be
changed.
The pressure curve up to maximum pressure appears in the display in the
form of a bar chart. By pressing the
key briefly, a lower pressure
can also be obtained.
03_Bedien_en.fm Seite 22 Mittwoch, 27. September 2006 5:12 17