Eppendorf FemtoJet - Manual (Page 20)

18
3 Mode of operation
3.3 Connecting the capillary holder
Universal capillary holder
The universal capillary holder is used to locate the injection capillaries.
Prefabricated Eppendorf Femtotips

/ Femtotips

II (6) are screwed into
the universal capillary holder using the adapter (5). To do so, the adapter
for Femtotips

(5) is first screwed into the front rotatable knurled screw
(4) and then the Femtotip

/ Femtotip

II is attached. If self-pulled
capillaries are to be attached, the grip head 0 (7) needs to be screwed
loosely into the front rotatable knurled screw (4). The self-pulled capillary
is pushed into the front opening of the grip head through the two O-rings
and fixed in position by tightening the grip head.
Universal capillary holder
1 Tube connection
2 Rear knurled screw
3 Tension piece
4 Front knurled screw, rotatable
5 Adapter for Femtotips

(microcapillaries for microinjection)
6 Femtotip

/ Femtotip

II
7 Grip head 0, plastic, with two O-rings and sealing washer
Connecting the universal capillary holder
The two-meter-long injection tube is connected to the switched on
FemtoJet

(1) via the bayonet joint (2). The injection tube (3) is screwed
to the rear knurled screw (4) of the universal capillary holder. Femtotips

/
Femtotips

II are fitted at the front end of the universal capillary holder (5)
or independently-manufactured capillaries are clamped with the aid of the
appropriate grip head.
1 2 3 4 5 6
7
1 2 3 4 5
03_Bedien_en.fm Seite 18 Mittwoch, 27. September 2006 5:12 17