Luminex xPONENT for MAGPIX - Manual (Page 78)

Single Step - Allows analysis of one well at a time. Select or clear this check box to turn this
function on or off.
Scroll Arrows - Use the up, down, left, and right arrow buttons to scroll through the table.
Maximize/Minimize - Use this toggle button to maximize and minimize the Plate Image, Dot
Plot, and List.
Plate - Select a plate from the drop-down list.
Save Image - Saves the information displayed on this page as an image file.
Progress - Click this button to display the Current Batch Run screen and to view real-time
batch processing results.
Analyze Current Batch
When you run a batch, the Current Batch tab opens. Here, you can view real-time analysis
of acquired analytes. Use this tab to view results, statistics, and log information related to the
current batch, and to perform statistical analysis on batch results. To view a single statistic
type for all analytes, click the statistic you want to view in the Statistics list. To view all
statistic types for a single analyte, click the analyte in the Analyte list. This tab offers real-
time monitoring of batch sampling during acquisition through a display of sample bead
statistics and analytes. The statistics available on this tab are intra-well bead statistics. They
do not describe replicate well assay results.
Statistic - To view a particular statistic for analytes in a batch, select one of the following:
• Median - The point in a data set where there are as many values occurring above it as
there are below it.
• Test Result - The calculated analysis value for quantitative or qualitative assays derived
from standards with known values.
• Range - A semi-quantitative result for a particular numerical result falling between a
predefined set of values such as “Normal” or “Negative”.
• Net MFI (Median Background) - Net MFI median background.
• Mean - Average of all values in a set of results.
• % CV of microspheres - The measure of relative dispersion within the distribution.
% CV = 100 x Std Dev / Mean
• Standard Deviation - For calculating sample variability or dispersion, Luminex uses the
standard deviation formula.
• Peak - The value that is equal to the largest number of data points within the distribution.
For example, in data set {1,2,2,3,3,3,4,5}, 3 is the peak because it occurs the largest
number of times in the distribution list.
• Trimmed Count*
• Trimmed Mean*
• Trimmed % CV of microspheres*
• Trimmed Standard Deviation*
• Trimmed Peak*
• % CV of Replicates - The measure of relative dispersion within the distribution of results
for replicate samples.
% CV = 100 X Std Dev / Mean
Using xPONENT
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