Luminex xPONENT for MAGPIX - Manual (Page 17)

NOTE: Refer to the product information sheet that accompanies your IVD
assay for additional information.
CAUTION: Do not use strong organic solvents with MAGPIX. For
information about specific compatibility, visit the Luminex
Technical Support website at http://www.luminexcorp.com/
support/faqs.html.
Safety Precautions
WARNING: All samples should be regarded as potentially contaminated and
treated as infectious. Samples should be handled at the
Biosafety Level 2, as recommended for any potentially
infectious human serum or blood specimen in the Center for
Disease Control/National Institutes of Health Manual, “Biosafety
in Microbiological and Biomedical Laboratories,” 1984.
WARNING: Although beads do not contain hazardous or carcinogenic
components at toxic levels, they may be toxic if swallowed. In
addition, contact with acids liberates toxic gases. If beads come
into contact with skin, wash immediately with copious amounts
of water. In case of an accident, seek medical advice
immediately and show the product label or container to your
medical provider. A material safety data sheet is available upon
request.
WARNING: The Drive Fluid and the solution in which MagPlex beads are
stored contain ProClin
® , which may cause an allergic reaction.
Use personal protective equipment, including gloves and safety
glasses. Check your assay's package insert for assay
component information.
Bead Concentration
Follow the instructions in the IVD kit’s product insert and use the provided software protocol.
MAGPIX Technology
The MAGPIX system operates by using magnetic beads (microspheres) that are coated with
a reagent specific to a particular bioassay, enabling the capture and detection of specific
analytes from a sample. The sample mixture is aspirated by the sample probe and conveyed
via Drive Fluid into the camera chamber, where the beads are pulled down into a monolayer
by the magnet, and immobilized, and imaged. Within the chamber, beads are exposed to a
red LED and a green LED, which excite both the internal dyes that identify each bead’s color
signature and the reporter fluorescence from the surface of the beads. The red LED is
responsible for classifying the beads. The CL1 and CL2 filters function to categorize the
beads based on color signature and place them properly on the bead map as well as throw
out any doublets that may exist.The green LED with the RP1 filter produces the reporter
fluorescence which identifies the analytes captured during the assay. The beads are then
flushed to the waste container, clearing room for the next sample.
Calibration is important to ensure that the optical system functions effectively and that
different Luminex MAGPIX systems report similar results. Calibrating the MAGPIX system
xPONENT for MAGPIX
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