Luminex xPONENT for MAGPIX - Manual (Page 19)

Classification and Reporter Fluorochromes
MagPix beads in the calibration kit are used to autofocus the camera and calibrate the CL1,
CL2, and RP1 channels. The beads in the verification kit are a mix of 6 different regions that
cover the range of the 50-plex map. Both calibration and verification beads are triple-dyed,
and the fluorescence signal of these dyes enables classification of each bead set.
TABLE 1. MAGPIX Active Bead Regions (by Region)
Region Region Region
MC10012 MC10013 MC10014
MC10015 MC10018 MC10019
MC10020 MC10021 MC10022
MC10025 MC10026 MC10027
MC10028 MC10029 MC10030
MC10033 MC10034 MC10035
MC10036 MC10037 MC10038
MC10039 MC10042 MC10043
MC10044 MC10045 MC10046
MC10047 MC10048 MC10051
MC10052 MC10053 MC10054
MC10055 MC10056 MC10057
MC10061 MC10062 MC10063
MC10064 MC10065 MC10066
MC10067 MC10072 MC10073
MC10074 MC10075 MC10076
MC10077 MC10078
Fluidics 1 and Fluidics 2
Although it undergoes a wash step in between wells, the probe can be susceptible to carry-
over from well-to-well. Fluidics 1 contains one bead set. Fluidics 2 contains a buffer solution
and a different control bead. The function of this maintenance procedure is to measure how
much (as a percentage) of the first bead set in Fluidics 1 is found in the well where Fluidics 2
has been loaded.
Sample Volume
Sample volumes range in size from 20 µL to 200 µL. Ensure that approximately 25 µL more
than the sample volume remains in the well after aspiration. This amount may vary
depending on the type of plate used. Your sample volume must be large enough to prevent
aspirating air into the fluid line when acquiring sample, and small enough to prevent spill-over
xPONENT for MAGPIX
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